Evaluation of the antioxidant activity of various extracts of aerial parts of Cassia absus: An in-vitro techniques

Abstract

Cassia absus (Linn) (family Fabaceae ) is generally known as “chaksu ” inan ayurvedic traditional system. The current study, aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Cassia absus, was evaluated for its in-vitro antioxidant potential by Diphenylpicrylhydrazyl radical, nitric oxide activity and total antioxidant activity taking ascorbate as the standard for all the three methods. The IC₅₀ value was originated that methanolic concentrates of Cassia absus more efficient in Diphenylpicrylhydrazyl radical, nitric oxide activity, total antioxidant activity compared EA&PE concentrates.The methanolic concentrates of Cassia absus & ascorbic acid exhibited antioxidant potential possessing IC₅₀230µg/ml &130µg/ml (Nitric oxide). 205µg/ml &57µg/ml (total antioxidant),195µg/ml & 66µg/ml (Diphenylpicrylhydrazyl radical) respectively. The difference in the scavenging potential of the extracts can be due to variation in the percentage of bioactive compounds present in different solvents. In vitro antioxidant studies obviouslyshow the methanolic concentrates of Cassia absushave better antioxidant activity. This result indicates that aerial parts of methanolic concentrates Cassia absuscould serve as a natural antioxidant, which may be useful in prevent free radical-induced diseases.