Deciphering the cytotoxic activity of Annona squamosa iron oxide nanoparticles against selective cancer cell line

Hemalatha S; Vanitha V; Pushpa Bharathi N; Jayalakshmi M; Amudha P; Mohanasundaram S

Hemalatha S ⁽¹⁾ , Vanitha V ⁽²⁾ , Pushpa Bharathi N ⁽³⁾ , Jayalakshmi M ⁽⁴⁾ , Amudha P ⁽⁵⁾ , Mohanasundaram S ⁽⁶⁾

(1) Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai-117, Tamil Nadu, India, India ,

(2) Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai-117, Tamil Nadu, India, India ,

(3) Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai-117, Tamil Nadu, India, India ,

(4) Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai-117, Tamil Nadu, India, India ,

(5) Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai-117, Tamil Nadu, India, India ,

(6) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam- 603308, Kanchipuram District, Tamil Nadu, India, India

Issue
Vol. 8 No. 2 (2017): Volume 8 Issue 2

Keywords:

Annona squamosal, nanoparticles, liver cancer, melanoma, iron oxide nanoparticles, MTT assay, cytotoxicity assay

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Abstract

In this present investigation, using hydroethanolic extract of leaves of Annona squamosal, iron oxide nanoparticle was synthesized at 600 c temperature and detected by UV–Visible spectrophotometer between 300 to 700 nm. The structure of nanoparticles was observed by SEM and FT-IR was performed to know the major functional groups. In anticancer assay, Fe3O4 NPs showed significant cytotoxicity on HepG2 and melanoma A375 cell lines. At the same time, Fe3O4 NPs showed zero toxicity on normal liver cell line. From our results, we could observe, this as a eco-friendly and nontoxic bio-reductant for the synthesis of Iron oxide nanoparticle with potential for cancer therapy.