Optimization of production of Extracellular proteolytic keratinase using Streptomyces sp. isolated from poultry plant soil

Mohanasundaram S; Doss VA; Prabhaharan CTA; Joseph J; Venugopal K; Agilandeshwari P; Sudansuhaa C; Balaganesh R; Ramkumar R; Rajaruban MDS; Balaji B

Mohanasundaram S ⁽¹⁾ , Doss VA ⁽²⁾ , Prabhaharan CTA ⁽³⁾ , Joseph J ⁽⁴⁾ , Venugopal K ⁽⁵⁾ , Agilandeshwari P ⁽⁶⁾ , Sudansuhaa C ⁽⁷⁾ , Balaganesh R ⁽⁸⁾ , Ramkumar R ⁽⁹⁾ , Rajaruban MDS ⁽¹⁰⁾ , Balaji B ⁽¹¹⁾

(1) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(2) Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India, India ,

(3) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(4) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(5) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(6) Department of Biotechnology, Vivekananda College of Engineering for Women, Tiruchengode, Tamil Nadu, India, India ,

(7) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(8) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(9) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(10) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India ,

(11) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam, Tamil Nadu, India, India

Issue
Vol. 8 No. 3 (2017): Volume 8 Issue 3

Keywords:

fermentation, Proteolytic keratinase, Streptomyces sp, stability

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Abstract

In this study, Proteolytic keratinase was observed from Streptomyces sp., isolated from poultry plant soil and purified by ammonium sulfate fractionation method followed by Gel filtration chromatography. The purified enzyme was further characterized partially which covers the influence of pH, temperature, incubation time, nitrogen sources and metal ions on the activity of proteolytic keratinase. From the results, it was observed that, proteolytic keratinase isolated from the Streptomyces sp. enumerated from the poultry plant soil showed maximal activity at pH 8.0 and at temperature 50oC within 55mins. It was also found that, at the minimum concentration of 2mM BaCl.2H2O, CuSO4.5H2O and MgCl2, it showed highest keratinase activity.