Isolation of Terflavin B from fruits of Terminalia chebula retz

Abstract

Terminalia chebula Retz is known as “Haritaki” belong to the family Combretaceae. It is also known as Mother of all healings. Literature survey reveals that it have antifungal, antibacterial, anticarcinogenic, antidiabetic, antipro- liferative, antiarthritic, anticaries and also multiprotective effect such as hepato-protective, pulmonary-protective, renal-protective, radio-protective, cytoprotective and cardio-protective effects. The present study investigates the phytochemical studies and structural elucidation of the medicinally active constituents in methanolic extract of fruits of Terminalia chebula Retz. The phytochemical analysis of Terminalia chebula Retz shows the presence of tannins, alkaloids, proteins, carbohydrates and phyto sterols were identified. Thin layer chromatography and High- performance thin layer chromatography analysis were confirmed the presence of tannins in methanolic extract of Terminalia chebula Retz. Column chromatography was used to isolate the compound and the structure was elucidated with spectroscopic methods. The Infrared spectroscopy, ¹H &¹³C Nuclear magnetic resonance spectroscopy and Mass spectroscopy analysis confirms the isolated compound was Terflavin B, A type of ellagi tannin which belongs to the hydrolysable tannins. The isolated compound was confirmed as Terflavin B. Terflavin B was reported an effective drug for the treatment of cancer and HIV disease. Thus the isolated compound may be synthesized in laboratory and can be applied for the treatment of the cancer diseases and prevention of HIV replication.