Characterization and anticancer efficacy of tamoxifen citrate nanosuspension against MCF-7 breast cancer cells

Abstract

Nanotechnology is one of the important fields that developed strategies to solve the issue of Tamoxifen (TAM) toxicity, owing to the capacity of nano-enabled-formulations to deliver smaller concentrations of TAM to cancer cells, over a longer period of time. Many different TAM-containing-nano systems have been successfully fabricated to selectively deliver TAM to particular molecular targets on tumour membranes, decreasing unwanted toxic effects. The present study was carried out to synthesis the tamoxifen citrate nanosuspension and also to find its effectiveness against breast cancer cells. Nanoparticles were made using multiple emulsion solvent evaporation method and its characterizations were studied. The structure and morphological examination of tamoxifen citrate Liquid self-nanoemulsifying drug delivery systems were observed with transmission electron microscopy. The cytotoxic effect of tamoxifen citrate L-SNEDDS against breast cancer MCF-7 cells was measured by cell viability assay. The cytotoxic effect of tamoxifen citrate L-SNEDDS enhanced significantly as the concentration of drug enhanced from 25 µM  to 250 µM. The toxic action of tamoxifen citrate L-SNEDDS on cancer cells remarkably reduced cell viability from 100% to 20.3%, while nanosuspension devoid of the drug presented cell viability of 99.5%. A significant decrease in cell viability was found in tamoxifen citrate free drug from 100% to 30.9%. In conclusion, tamoxifen citrate Liquid self-nanoemulsifying drug delivery systems showed cytotoxic activity on MCF-7 breast cancer cell lines.