UNI-Directional Double Run Multi-Marker Based Standardization of “Amruthothram” Ayurvedic Medicine by HPTLC

Abstract

Ayurveda and Unani Medicine are the most classy and commonly practiced systems in India. In India’s AYUSH systems, some 8,000 herbal remedies have been codified. Amruthotharam/Amrutottaram Kashayam is one of such preparation which takes care of metabolic disorders through inflammation. The formulation contains guduchi (Tinospora cordifolia), haritaki (Terminalia chebula), and shunthi (Zingiber officinale) in the ratio 6:4:2. Natural remedies obtained from wild sources; therefore, sustaining consistent product quality is difficult because of extrinsic variables including soil conditions, light and water availability, temperature changes, nutrients, and geographic location. The present work aimed to develop and validate uni-dimensional double development high-performance thin layer chromatography to standardize the marker-based compounds such as gallic acid, berberine and gingerol-6, because the power of one-dimensional chromatography is often inadequate for complete resolution of the components present in complex samples which can be improved by separating actives through UDDD.