Differential expression of glycogen synthase kinase 3 Alpha and 3 Beta isomers in brain cortex of mice following high doses of glucose

Mohd Alaraj (1) , Irena Kosinska (2) , Bahaa Deen Al-Trad (3) , Ammar Almaaytah (4) , Tarek D. Hussein (5) , Ashfaque Hossain (6) , Mohamed Jamal Saadh (7)
(1) Department of Pharmacology, College of Medicine, University of Hail, Kingdom of Saudi Arabia, Saudi Arabia ,
(2) Department of Social Medicine and Public Health, Medical University of Warsaw, Poland, Poland ,
(3) Department of Biological Sciences, Yarmouk University, Irbid, Jordan, Jordan ,
(4) Department of Pharmaceutical Technology, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan, Jordan ,
(5) Department of Zoology, Faculty of Science, Cairo University, Egypt, Egypt ,
(6) Department of Medical Microbiology, Ras Al Khaimah Medical and Health Sciences University, Ras Al Khaimah, UAE, United Arab Emirates ,
(7) Department of Pharmacy, Faculty of Pharmacy, Middle East University, Amman, Jordan, Jordan

Abstract

Glycogen synthase kinase 3 (GSK3) encodes a serine/threonine protein kinase. We investigated the effects of Subcutaneous (SC) glucose administration on the expression of glycogen synthase kinase 3 (GKS-3) isomers (α and β) genes in the cerebral cortex of mice with the aim of determining the possible mechanism(s) involved in mitochondrial dysfunction induced by hyperglycemia. Adult male BALB/c mice were treated with 12 gm/kg glucose solution SC once daily for 3 days. Ultrastructure study, histopathological analysis, and Real-time PCR investigations were carried out on the cerebral cortex from glucose treated mice and from vehicle-treated control animals. We observed significant ultrastructural damage of mitochondria in the cerebral cortex of mice received high doses of glucose. Histopathological alterations in the cortex of these animals were also detected. A significant increased of GSK-3α gene expression and decreased expressions of GKS-3β gene in high glucose treated animals were noticed. The hyperglycemia-induced ultrastructural changes may occur via modulation of gene expression of GSK-3 isomers, and we hypothesize this as an early etiopathological factor in hyperglycemia-related neurodegenerative diseases (NDD).

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Authors

Mohd Alaraj
malaraj@meu.edu.jo (Primary Contact)
Irena Kosinska
Bahaa Deen Al-Trad
Ammar Almaaytah
Tarek D. Hussein
Ashfaque Hossain
Mohamed Jamal Saadh
Mohd Alaraj, Irena Kosinska, Bahaa Deen Al-Trad, Ammar Almaaytah, Tarek D. Hussein, Ashfaque Hossain, & Mohamed Jamal Saadh. (2020). Differential expression of glycogen synthase kinase 3 Alpha and 3 Beta isomers in brain cortex of mice following high doses of glucose. International Journal of Research in Pharmaceutical Sciences, 11(1), 993–999. Retrieved from https://ijrps.com/home/article/view/701

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