In vitro antifungal study of green synthesized silver nanoparticles from Acacia nilotica leaves extract against a plant and human pathogens

Magesh R; Sivakumar K; Karthikeyan V; Mohanasundaram S; Subathra M; Joseph J; Venugopal K; Sasikala R

Magesh R ⁽¹⁾ , Sivakumar K ⁽²⁾ , Karthikeyan V ⁽³⁾ , Mohanasundaram S ⁽⁴⁾ , Subathra M ⁽⁵⁾ , Joseph J ⁽⁶⁾ , Venugopal K ⁽⁷⁾ , Sasikala R ⁽⁸⁾

(1) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India ,

(2) Department of Biomedical Engineering, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India ,

(3) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India ,

(4) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India ,

(5) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India ,

(6) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India ,

(7) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India ,

(8) Department of Biotechnology, Karpaga Vinayaga College of Engineering and Technology, Maduranthagam-603308, Kanchipuram District, Tamil Nadu, India, India

Issue
Vol. 10 No. 1 (2019): Volume 10 Issue 1

Keywords:

Antifungal, Nanoparticles, Acacia nilotica, Silver

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Abstract

Comparing to other nanoparticles, Silver nanoparticles have attained a special place in the field of nanotechnology due to their wider applications. This study was about to evaluate the green synthesized AgNPs for their effective Antifungal potential. The objective of this study was to evaluate the Antifun- gal efficacy of AgNps by using in vitro studies. In this investigation, aqueous extract of Acacia nilotica leaves was used as a reducing and stabilizing agent for the eco-friendly synthesis of silver nanoparticles. The Bio-reduction and stabilization of formed AgNps were monitored by UV-visible spectrophotometer. The surface Plasmon resonance of these particles was approximately 430 nm. The Structural, Optical and morphological properties of the synthesized AgNps were characterized by using SEM with EDAX and FTIR. The phytochemical analysis of Acacia nilotica leaves extract had also performed using various methods. The Anti-fungal activity of Plant extracts and synthesized AgNps were tested against five fungal pathogens such as Fusarium oxysporium, Aspergillus niger, Trichoderma reesei, Candida albicans and Rhizopus stolonifer using well diffusion method.