UV Spectrophotometry and FTIR analysis of Phenolic Compounds with Antioxidant Potentials in Glycyrrhiza glabra and Zingiber officinale
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Glycyrrhiza Glabra, Zingiber Officinale, Flavonoids, Glycyrrhizic Acid, Gingerol
Abstract
Notable growth in the usage of herbal remedies could be seen in recent days as an effective alternative choice of drugs. In particular, phenolic compounds have gained the highest attention and priority among the scientific community in exploring them as a novel drug for a wide range of communicable and non-communicable diseases. It has been studied well and proven extensively that the antioxidant potential of plants is largely depending on its phenolic contents. This study was focused to compare and analysis the biochemical composition, antioxidant activity and characterization of phenolic compounds in Glycyrrhiza glabra and Zingiber officinale root extracts. The ethanolic and chloroform extracts of Glycyrrhiza glabra and Zingiber officinale roots were subjected to preliminary screening. The qualitative Phytochemical tests of the Glycyrrhiza glabra and Zingiber officinale root showed the existence of polyphenols, alkaloids, saponins, terpenoids, flavanoids, amino acids and proteins. The DPPH assay of Glycyrrhiza glabra and Zingiber officinale root tissues showed a significant antioxidant property. FT-IR and UV Spectral studies of Glycyrrhiza glabra and Zingiber officinale roots showed the presence of different biologically significant flavonoids. The results of this study exemplifies that the selected plants are notable sources of phenolic compounds and hence observed that significant antioxidant potential.
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References
Al-Qarawi, A. A., et al. 2001. Hepatoprotective Activity of Licorice in Rat Liver Injury Models. Journal of Herbs, Spices & Medicinal Plants, 8(1):7–14.
Amiril, N. A. 2006. Optimization of essential oil extraction from Zingiber officinale. pages 1–65. (Doctoral dissertation, Kuktem).
Ammosov, A. S., Litvinenko, V. I. 2003. Triterpenoids of plants of glycyrrhiza L. and meristotropis fisch. et mey genuses (a review). Pharmaceutical Chemistry Journal, 37(2):83–94.
Arystanova, T. P., et al. 2001. Chromatographic determination of glycyrrhizinic acid in Glycyrrhiza glabra preparation. Chemistry of Natural Compounds, 1(37):89–90.
Atmani, D., et al. 2009. Flavonoids in Human Health: From Structure to Biological Activity. Current Nutrition & Food Science, 5(4):225–237.
Baker, F. 1988. Determination of serum tocopherol by colorimetric method. Varley’s practical clinical biochemistry. page 902, Portsmouth, NH, USA. Portsmouth, NH, USA: Heinemann Professional Publishing.
Beauchamp, C., Fridovich, I. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44(1):276–287.
Beers, R. F., Sizer, I. W. 1952. A Spectrophotometric Method For Measuring The Breakdown Of Hydrogen Peroxide By Catalase. Journal of Biological Chemistry, 195(1):133–140.
Bhattarai, S., et al. 2001. The Stability of Gingerol and Shogaol in Aqueous Solutions. Journal of Pharmaceutical Sciences, 90(10):1658–1664.
Blaschek, W., et al. 2013. Hagers Handbuch der Pharmazeutischen Praxis: Folgeband 2: Drogen A- K (German). page 909. Springer; 5. Aufl.
Cinatl, J., et al. 2003. Glycyrrhizin, an active component of liquorice roots, and replication of SARS-associated coronavirus. The Lancet, 361(9374):2045–2046.
Curreli, F., et al. 2005. Glycyrrhizic acid alters Kaposi sarcoma–associated herpesvirus latency, triggering p53-mediated apoptosis in transformed B lymphocytes. Journal of Clinical Investigation, 115(3):642–652.
De Clercq, E. 2000. Current lead natural products for the chemotherapy of human immunodeficiency virus (HIV) infection. Medicinal research reviews, 20(5):323–349.
Doss, V. A., et al. 2016. Analysis of hydroethanolic extract of Senna alata (L.) to screen bioactive compounds with inhibitory activity on lipid peroxidation, in vitro antibacterial and antidiabetic efficacy. Int J Pharma Sci, 6(1):1360–1366.
Elattar, T. M., Virji, A. S. 2000. The inhibitory effect of curcumin, genistein, quercetin and cisplatin on the growth of oral cancer cells in vitro. Anticancer research, 20(3A):1733–1738.
Gryglewski, R. J., et al. 1987. On the mechanism of antithrombotic action of flavonoids. Biochemical Pharmacology, 36(3):317–322.
Harborne, J. B. 1973. Phytochemical Methods. pages 49–188. Chapman and Hall Ltd., London.
Harikrishnan, R., et al. 2011. Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture, 317(1- 4):1–15.
Kaur, R., et al. 2013. Glycyrrhiza glabra: a phytopharmacological review. International journal of pharmaceutical Sciences and Research, 4(7):2470– 2477.
Khattak, K. F., Simpson, T. J. 2010. Effect of gamma irradiation on the antimicrobial and free radical scavenging activities of Glycyrrhiza glabra root. Radiation Physics and Chemistry, 79(4):507–512.
Kumari, A., Kumar, J. 2019. Phytochemical screening of root extracts of Glycyrrhiza glabra by spectroscopic methods (UV-VIS spectrophotometer, FTIR and HPLC). Int. J. Pharmaceut. Sci. Drug Res, 11(6):376–381.
Lin, J. Y., Tang, C. Y. 2007. Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101(1):140–147.
Mabry, T. J., et al. 1970. The Ultraviolet Spectra of Flavones and Flavonols. In: The Systematic Identification of Flavonoids. pages 41–164, Berlin Heidelberg. Springer, Berlin, Heidelberg.
Maqsood, S., et al. 2011. Emerging role of immuno stimulants in combating the disease outbreak in aquaculture. International Aquatic Research, 3(3):147–163.
Matasyoh, L. G., et al. 2007. Chemical composition and antimicrobial activity of the essential oil of Ocimum gratissimum L. growing in Eastern Kenya. African journal of biotechnology, 6(6):760–765.
Medoua, G. N., et al. 2009. Nutritional value and antioxidant capacity of lunch meals consumed by elderly people of Sharpeville, South Africa. Food Chemistry, 115(1):260–264.
Omaye, S. T. 1979. Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods in enzymology, 62:3–11.
Parekh, J., Chanda, S. 2007. In vitro antibacterial activity of the crude methanol extract of Woodfordia fruticosa Kurz. flower (Lythraceae). Brazilian Journal of Microbiology, 38(2):204–207.
Rehman, R., et al. 2011. Zingiber officinale Roscoe (pharmacological activity). Journal of Medicinal Plants Research, 5(3):344–348.
Sohi, K. K. 2003. Gallic Acid, an Antioxidant, Exhibits Antiapoptotic Potential in Normal Human Lymphocytes: A Bcl-2 Independent Mechanism. Journal of Nutritional Science and Vitaminology, 49(4):221–227.
Sugumar, M., et al. 2019. Pharmacological analysis of hydroethanolic extract of Senna alata (L.) for in vitro free radical scavenging and cytotoxic activities against HepG2 cancer cell line. Pakistan Journal of Pharmaceutical Sciences, 32(3):933–936.
Visavadiya, N. P., et al. 2009. Evaluation of antioxidant and anti-atherogenic properties of G- lycyrrhiza glabra root using in vitro models. International Journal of Food Sciences and Nutrition, 60(sup2):135–149.
Wang, Z. Y., et al. 2000. Licorice in foods and herbal drugs: Chemistry, pharmacology, toxicology and uses. pages 321–353. Herbs, Botanicals & Teas. Technomic Publishing Co. Inc, Lancaster.
Zhou, H., et al. 1998. Analysis of essential oil from rhizoma Zingiberis by GC-MS. Zhongguo Zhong Yao Za Zhi, 23(4):234–236.
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