Pilot scale studies on the optimization of DIVA assay for the inactivated whole cell paratuberculosis vaccine

Sujata Jayaraman; Kundan Kumar Chaubey; Shoor Vir Singh; Gajendra Kumar Aseri; Mukta Jain; Saurabh Gupta; Manju Singh; Parul Yadav; Neeraj Khare; Deepansh Sharma; Jagdip Singh Sohal

Sujata Jayaraman ⁽¹⁾ , Kundan Kumar Chaubey ⁽²⁾ , Shoor Vir Singh ⁽³⁾ , Gajendra Kumar Aseri ⁽⁴⁾ , Mukta Jain ⁽⁵⁾ , Saurabh Gupta ⁽⁶⁾ , Manju Singh ⁽⁷⁾ , Parul Yadav ⁽⁸⁾ , Neeraj Khare ⁽⁹⁾ , Deepansh Sharma ⁽¹⁰⁾ , Jagdip Singh Sohal ⁽¹¹⁾

(1) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India ,

(2) ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, India, India ,

(3) ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, India, India ,

(4) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India ,

(5) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India ,

(6) ICAR-Central Institute for Research on Goats, Makhdoom, Mathura, India, India ,

(7) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India ,

(8) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India ,

(9) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India ,

(10) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India ,

(11) Amity Center for Mycobacterial Disease Research, Amity Institute of Microbial Technology, Amity University Rajasthan, Jaipur, India, India

Issue
Vol. 9 No. 2 (2018): Volume 9 Issue 2

Keywords:

Camptothecin, Carotenoid, Cellulase Enzyme, Extraction, HPLC

PDF

Abstract

Paratuberculosis or Johne’s disease is chronic enteritis of domestic ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). Paratuberculosis results in substantial losses to livestock industry and is also threat to human health, since MAP has zoonotic concerns with Crohn’s disease. Therefore, control of this disease is priority to governments. However, widely used ‘Test and Cull’ control programs have yielded little or no success and alternate approaches are required to control this disease. Vaccination has shown great promise in managing this disease, but there are regulatory restrictions on use of vaccination due to lack of DIVA tool (differentiation of infected and vaccinated animals). In the present study, we report the development of first serology based DIVA tool for the inactivated paratuberculosis vaccine using unique biomarkers present in the secretome of MAP that are not part of vaccine.