Apigenin reduces cell viability of Human epidermoid cancer cells (Hep-2)

Shanmugam Manoharan; Tharmaraj Rejitharaji; Murugaraj Manoj Prabhakar; Sekar Karthikeyan; Villianur Ibrahim Hairul Islam

Shanmugam Manoharan ⁽¹⁾ , Tharmaraj Rejitharaji ⁽²⁾ , Murugaraj Manoj Prabhakar ⁽³⁾ , Sekar Karthikeyan ⁽⁴⁾ , Villianur Ibrahim Hairul Islam ⁽⁵⁾

(1) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India, India ,

(2) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India, India ,

(3) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India, India ,

(4) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India, India ,

(5) Pondicherry Centre for Biological Sciences, Boomyinpet, Puducherry-5, India, India

Issue
Vol. 5 No. 3 (2014): Volume 5 Issue 3

Keywords:

Apigenin, apoptosis, cell viability, Hep-2

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Abstract

The aim of the present study is to explore the cytotoxic potential of apigenin in Hep-2 cells. The mechanistic pathway for its cytotoxic potential was analyzed using MTT assay (cytotoxicity), DNA fragmentation (agarose gel electrophoresis), Nuclear damage (DAPI Staining), ROS generation ability (DCFH-DA method), apoptosis inducing potential (dual staining) and expression of apoptosis and angiogenic proteins (Western blotting). The overall findings of the present study suggest that apigenin might have reduced the cell viability of Hep-2 cells through its apoptotic and anti-angiogenic potential.

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Shanmugam Manoharan

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India

sakshiman@rediffmail.com (Primary Contact)

Tharmaraj Rejitharaji

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India

Murugaraj Manoj Prabhakar

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India

Sekar Karthikeyan

Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar – 608 002, Tamil Nadu, India

Villianur Ibrahim Hairul Islam

Pondicherry Centre for Biological Sciences, Boomyinpet, Puducherry-5, India

Shanmugam Manoharan, Tharmaraj Rejitharaji, Murugaraj Manoj Prabhakar, Sekar Karthikeyan, & Villianur Ibrahim Hairul Islam. (2014). Apigenin reduces cell viability of Human epidermoid cancer cells (Hep-2). International Journal of Research in Pharmaceutical Sciences, 5(3), 153–159. Retrieved from https://ijrps.com/home/article/view/3801

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Apigenin reduces cell viability of Human epidermoid cancer cells (Hep-2)

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