Application of Planar chromatography for the simultaneous determination of Metformin Hydrochloride and Glimepiride in combined dosage form

Abstract

A Simple, sensitive, selective and precise high-performance thin layer chromatographic method for analysis of Metformin Hydrochloride and glimepiride in combined dosage form. The method employed TLC aluminium plates Precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted of toluene: methanol: ethyl acetate: formic acid (3:6:3:0.2, v/v/v/v). This system was found to give compact spots for Metformin Hydrochloride and glimepiride (R_f value of 0.74 ± 0.01 and 0.08 ± 0.01). Densitometric analysis of Metformin Hydrochloride and glimipiride were carried out in the absorbance mode at 216 nm. The linear regression data for the calibration plots showed good relationship with, r² = 0.9975 ± 0.01 from 200 -1400 ng for Metformin Hydrochloride and r² = 0.9999 ± 0.02 from 20-140 ng for glimipiride, respectively. The methods were validated for precision, accuracy, ruggedness and recovery. The limits of detection and quantification were 50 and 100 ng per spot for Metformin Hydrochloride and 5 and 50ng per spot for glimipiride, respectively.