Antidiabetic effect of 2-(4- [(2-hydroxyacetyl benzyl) ketoamino]-phenyl amino-methyl)- hydrobenzophenon on Alloxan induced rats

Abstract

Diabetes Mellitus is known as a syndrome, a collection of disorders with high blood glucose level & glucose intolerance as its feature, either because of insulin deficiency or insulin impairment or both. Diabetes Mellitus, based on insulin, is broadly classified into two types. Type one indicates the distraction of pancreatic β - cells that leads to diabetes mellitus, in which insulin is necessary to prevent ketoacidosis, coma, death. Type two diabetes is characterized by disorders of insulin resistance & secretion. Male Wistar albino rats with an average weight of 180-250 g were used in this study. With a 12 hours’ light and dark period, they were kept under normal conditions (room temperature 24-27^oC and humidity 60-65 %). The free access of drinking water & pellet diet to male Wistar albino rats was allowed, as per the CPCSEA guidelines. Anti-diabetic activity of Compound 2-(4- [(2-hydroxyacetyl benzyl) ketoamino]-phenyl amino-methyl)- hydrobenzophenon, male Wistar albino rats were divided into four different groups. 1ml of blood samples were collected directly into anticoagulant bottles from the tail vein & later plasma was collected after centrifugation. Blood sugar levels were determined by spectrophotometer. There was a significant decrease in blood sugar levels in Alloxan + Glibenclamide and Alloxan + Compound groups on compared to control. The present self-funded study concludes that antidiabetic activity of 2-(4- [(2-hydroxyacetyl benzyl) ketoamino]-phenyl amino-methyl)- hydrobenzophenon in Alloxan induced diabetic rats significantly shows decreed blood sugar levels when compared to the control group.