Stability indicating RP-HPLC method for the simultaneous estimation of Quercetin and Rutin in bulk drug

Abstract

A stability-indicating HPLC technique has been developed and validated for Quercetin and Rutin under different stability environments of acid, base, neutral, oxidative, and photolytic degradation. The stability study were executed according to the ICH guidelines. The separation was accomplished utilizing Phenomenex Luna C₁₈  (250 x 4.6mm i.d, 5µ) column at a detection wavelength of 259 nm utilizing the mobile phase established of 20mM Ammonium acetate (pH 3.0) and acetonitrile 60: 40, v/v at the flow rate of 1.0 ml/min and injection volume of 20 µl. The linearity of the developed technique was obtained over the range of 1-5 µg/ml and 0.1-0.5 µg/ml for Quercetin and Rutin, respectively, with a correlation coefficient of more than 0.99. Further, the stress degradation studies were performed for which the results obtained stated that Quercetin and Rutin were more susceptible to acidic and oxidative conditions. The developed method provides good sensitivity and excellent reproducibility and can be further used to study the stability in formulations.