Phytochemical analysis and in vitro antioxidant screening of sea grass-Enhalus acoroides

Abstract

The present study was conducted to determine the yield of extract, qualitative, quantitative analysis and antioxidant activity of the crude extracts of leaves of Enhalus acoroides. The extracts were prepared by using Hexane, Chloroform, Ethanol, Ethyl acetate and Water solvents. The yield of extract for all the five solvents was calculated and they were studied for qualitative analysis of phytochemical compounds. The crude extracts were evaluated for total phenolic and total flavonoids contents. The antioxidant activity of crude extract of Enhalus acoroides was assessed by FRAP assay and the scavenging activity towards DPPH radical, H2O2, NO and ABTS. The present study revealed the presence of major phytochemicals like alkaloids, phenols, flavonoids, steroids, tannins, saponins. Quantitative analysis of the total phenols were high in Aqueous which has 78.36 mgGAE/g and total flavonoids were high in Aqueous which has 57.52 mgQU/g. Free radical scavenging activities was found high in Aqueous extract with maximum percentage of inhibition.