Process optimization for the cultivation of anti-cancer Pseudomonas aeruginosa (MTCC 647) bacterial strains

Abstract

Cancer is one of the most dangerous diseases worldwide. Bacterial products such as proteins and endotoxins (Lipo polysaccharides) have been tested for cancer treatment. In this work an effort was made to increase the biomass and total protein content of Pseudomonas aeruginosa extract by using Asparagine proline broth and optimum growth conditions. Bacterial strains of Pseudomonas aeruginosa MTCC no 647collected from MTCC, Chandigarh. AP broth was optimized by addition of copper sulphate, methylamine and aluminum sulphate. Modified Asparagine proline broth (AP1 to AP5) was prepared and the effect of graded amounts of copper sulphate and aluminum sulphate were analyzed. Asparagine proline broth supplemented with 0.03% of copper sulphate and 0.02 % of aluminum sulphate (AP3) was selected as it has shown high cell growth rate. Influences of various culture conditions such as temperature, PH, Incubation time on cultivation of Pseudomonas aeruginosa in modified Asparagine proline broth were studied. The highest protein production was achieved at 43ºC, at pH 8 for a period of 48hrs.