Evaluation of aqueous and hydro alcoholic extracts of Clerodendrum viscosum V. leaves & Macrotyloma uniflorum L. seeds against DMH-induced colon cancer

Abstract

Evaluation of anti-neoplastic activity of aqueous and hydro alcoholic extracts of Clerodendron viscosum V. leaves & Macrotyloma uniflorum L. seeds against DMH-induced colon cancer is the objective of this study. Extracts were obtained using cold maceration process. Six groups of animals, each containing ten male albino wistar rats (80 – 100 g) were administered with respective treatment to evaluate the anti-neoplastic activity. On the last day of experimental period, fecal matter was collected and colons were removed. Fecal matter was homogenized, and subjected to the measurement of fecal pH and bacterial enzymes. Colons of five animals were subjected for the presence of MPLs, ACFs in each colon, and histopathology by using standard methodology. Colons of remaining five animals were subjected to homogenization to measure the bacterial enzymes such as β-Glucosidase, β-Glucuronidase, and mucinase. The extracts showed significant protective effect by showing significant decrease in the levels of fecal and colon β-glucosidase, β-glucuronidase, mucinase; reduction in total number of ACFs in each colon and crypt multiplicity. Although, all the plant extracts has shown protective effect against colon carcinogenesis, treatment with hydro alcoholic extracts has produced more pronounced effect as compared with aqueous extracts.