Development and validation of Thin-Layer Chromatographic method for determination of α-mangostin in young pericarp, ripe pericarp and bark extract of Garcinia mangostana L. using TLC-­Densitometry

Abstract

A simple, fast and precise quantitative high performance thin-­layer chromatographic method has been developed for  quantitative  estimation  of  α-­mangostin  in  young  pericarp,  ripe  pericarp  and  bark  extract  of  Garcinia  mangostana L. (Hypericaceae). Determination was performed by using TLC plates with F254 silica gel as stationary phase and chloroform-­methanol (10: 0,1) as mobile phase. 2 μL of extract spotted on TLC plate, then eluted and analyzed  by  using  densitometry.  Concentration  of  α-­mangostin  was  determined  by  using  calibration  curve  from some  standard  concentrations  of  α-­mangostin.  This  study  performed  that  the  highest  concentration  of  α-­mangostin was in ripe pericarp (15,85%). Meanwhile, concentration of α-­mangostin in young pericarp was 4,19% and 3,88% in bark extract.