Lupeol protects DNA damage against 7,12-dimethylbenz(a)anthracene induced genotoxicity

Duraisamy Palanimuthu; Shanmugam Manoharan; Simon Silvan; Nagarethinam Baskaran; Ramachandran Srinivasan

Duraisamy Palanimuthu ⁽¹⁾ , Shanmugam Manoharan ⁽²⁾ , Simon Silvan ⁽³⁾ , Nagarethinam Baskaran ⁽⁴⁾ , Ramachandran Srinivasan ⁽⁵⁾

(1) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai nagar – 608002, Chidambaram, Tamil Nadu, India, India ,

(2) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai nagar – 608002, Chidambaram, Tamil Nadu, India, India ,

(3) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai nagar – 608002, Chidambaram, Tamil Nadu, India, India ,

(4) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai nagar – 608002, Chidambaram, Tamil Nadu, India, India ,

(5) Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai nagar – 608002, Chidambaram, Tamil Nadu, India, India

Issue
Vol. 3 No. 2 (2012): Volume 3 Issue 2

Keywords:

Lupeol, DMBA, Genotoxicity, Lipid Peroxidation, Antioxidants, Comet Assay

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Abstract

The present study has investigated the protective effect of lupeol on 7,12-dimethylbenz (a)anthracene (DMBA) induced genotoxicity in the bone marrow cells of golden Syrian hamsters. DNA damage was assessed in the bone marrow cells using single cell gel electrophoresis (comet assay) in experimental hamsters. Lipid peroxidation and antioxidants status in plasma was utilized as biochemical endpoints to assesses the antigenotoxic effect of lupeol during DMBA induced genotoxicity. Extensive DNA damage with marked abnormalities in the status of lipid peroxidation and antioxidants was noticed in hamsters treated with DMBA alone. Oral pretreatment of lupeol to DMBA-treated hamsters not only protected DNA damage, but also restored the status of lipid peroxidation and antioxidants. The present study thus suggests that lupeol exhibited antigenotoxic potential against DMBA-induced genotoxicity, which is probably due to its antioxidant property.