In vitro anti-inflammatory activity of hydroalcoholic extract of Asparagus racemosus roots

Abstract

Asparagus racemosus commonly known as Shatavari belonging to family Liliaceae. In India, it is found in Himalaya’s up to an altitude of 1300-1400m and all tropical part. Its various uses are galactogogue, tonic, diuretic, rheumatism, inflammation, nervine disorder etc. The aim of study is to evaluate anti-inflammatory activity of hydroalcoholic extract of Asparagus racemosus (ARHE) roots by using human red blood cell membrane stabilisation (HRBC) method and protein denaturation method. The inhibition of hypotonicity induced HRBC membrane lysis and denaturation of protein was taken as a measure of the anti inflammatory activity. The percentage of membrane stabilisation and protein denaturation for hydroalcoholic extracts and Diclofenac sodium were done at different concentrations. The maximum membrane stabilization and denaturation of protein of A. racemosus extracts was found to be 76.63 % at a dose of 1600 μg/ml and 545.91 at a dose of 2000 μg/ml respectively.