Naringin and 5-fluorouracil suppress inflammatory Cytokines in human skin cancer cell line

Deepa Suruli; Fathima Bushra Sheriff Mirza; Gloria Jemmi Christobel R; Amuthavalli Kottaiswamy; Shila Samuel; Vijayaraghavan Radhakrishnan

Deepa Suruli ⁽¹⁾ , Fathima Bushra Sheriff Mirza ⁽²⁾ , Gloria Jemmi Christobel R ⁽³⁾ , Amuthavalli Kottaiswamy ⁽⁴⁾ , Shila Samuel ⁽⁵⁾ , Vijayaraghavan Radhakrishnan ⁽⁶⁾

(1) Department of Biochemistry, VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India, India ,

(2) Department of Biochemistry, VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India, India ,

(3) Department of Biochemistry, VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India / Department of Biochemistry, VV Vanniaperumal College for Women, Virudhunagar, Tamil Nadu, India, India ,

(4) Department of Biochemistry, VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India, India ,

(5) Department of Biochemistry, VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India, India ,

(6) Department of Biochemistry, VRR Institute of Biomedical Science (Affiliated to University of Madras), Chennai, Tamil Nadu, India, India

Issue
Vol. 12 No. 1 (2021): Volume 12 Issue 1

Keywords:

Naringin, 5-fluorouracil, Inflammation, Cytokines, Anti-inflammatory

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Abstract

Naringin is a citrus flavonoid recently studied for anti-inflammatory activity in numerous cancer cells. In this study, the anti-inflammatory properties of naringin along with 5-fluorouracil in human skin cancer cell lines A375 was analyzed. A375 cells were treated with naringin, 5-fluorouracil alone, and combination. MTT assay and cell viability assays was demonstrated to detect the inhibitory effects of naringin or 5-fluorouracil on cell proliferation. mRNA expression of TNFα, IL-6, IL-1β, and NFκB were determined using quantitative RT-PCR. The effect of naringin and 5-fluorouracil combination significantly inhibited the growth and proliferation of the A375 cells in a concentration dependent manner with the IC50 values of naringin (24.75 µM) 5- fluorouracil (2.5 µM). The combination of naringin+5-fluorouracil on A375 cell lines at a concentration of half IC₅₀ values (12µM+1 µM). Naringin and 5-fluorouracil combination also decreased the level of TNFα, IL-6, IL-1β, and NFκB mRNA in the A375 cell line. Naringin and 5-fluorouracil exerted anti-inflammatory effect through the suppression of NF-kB, IL-1β, TNFα, IL-6 in A375 cells. Taken together, our results suggested that treating A375 with naringin and 5-fluorouracil combination may have future applications in treating skin cancers through its anti-inflammatory effect.

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