Green tea phytocompounds targets Lansterol 14-α demethylase against ergosterol biosynthesis in Candida glabrata

Priyanka Sirari; Jigisha Anand; Devvret; Ashish Thapliyal; Nishant Rai

Priyanka Sirari ⁽¹⁾ , Jigisha Anand ⁽²⁾ , Devvret ⁽³⁾ , Ashish Thapliyal ⁽⁴⁾ , Nishant Rai ⁽⁵⁾

(1) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,

(2) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,

(3) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,

(4) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India ,

(5) Department of Biotechnology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, India, India

Issue
Vol. 12 No. 3 (2021): Volume 12 Issue 3

Keywords:

Candida, Erg11, Ergosterol, Green Tea, Rutin

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Abstract

Green tea is credited as one of the world’s healthiest drinks with enriched antioxidants. It is known for its multi-beneficial health benefits against diabetes, blood pressure, hypertension, gastro-intestinal upset and is bestowed with significant antimicrobial potential. There are previous scientific evidence highlighting the antifungal potential of green tea and has identified it as a potential inhibitor of non-albicans Candida species. Lansterol 14-α demethylase (Erg 11) or CYP51 protein belongs to the cytochrome P450 monooxygenase (CYP) superfamily. Erg 11 is involved in ergosterol biosynthesis and has a significant role in azole drug resistance in Candida glabrata. The present study attempted to identify the inhibitory potential of green tea phytocompounds against inhibition of Erg 11 in Candida glabrata using bioinformatics tool viz., autodock vina software. Out of 15 green tea phytocompounds investigated, the study identified, Rutin (-10.5 kcal) Kaempferitrin (-9.4kcal), Epigallocatechin gallate (-10kcal), Epicatechin gallate (-8.7kcal), and Coumaroylquinic acid (-8.6kcal) acid as the potent phytocompounds which showed significant molecular interaction with Erg 11 in Candida glabrata. In attribution to the constant emergence of azole-resistant isolates, this preliminary analysis therefore, indicated the potential of green tea phytocompounds against inhibition of non-albicans Candida specific candidiasis. However, further, in vitro antimicrobial efficacy of these phytocompounds, the dose regime, drug likeliness, and cytotoxic analysis are required to be investigated and validated.

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