Evaluation of grape seed extract (Vitis vinifera) as a crosslinker on the stability of dentine collagen in total-etch adhesive systems: An in-vitro study

Abstract

The bond strength between resin-dentine is determined by the integrity of collagen, dentine and resin monomers. The susceptibility of the dentine bond results from the instability of the bonding structure that adheres to the bonding material. This can be achieved by using collagen cross-linker, synthetically and naturally in dentine substrates which are effective in protecting collagen fibrils from degradation, as proanthocyanidine. The aim of this study was to see the effect of grape seed extract as a crosslinker on the stability of dentine collagen and see the differences between groups. This study used 27 fresh premolars or third molars which were divided into 3 groups, namely group I giving grape seed extract 6.5%, group II giving chlorhexidine 2%, and group III only giving bonding ingredients. Samples were analyzed using SEM with 1000x magnification. Data was analyzed using the kappa statistic, Kruskal-Wallis, dan Mann-Whitney. The result of the study significant differences between treatment groups (P <0.05), the 2% chlorhexidine group showed the highest value compared to the grape seed extract group and without treatment. Grape seed extract can be used as an alternative to chlorhexidine as a crosslinker to maintain bone strength of the composite restoration.