Assessment of phytochemicals and quantification of primary and secondary metabolites of Artabotrys hexapetalus (L.f.) Bhandari leaves

Abstract

The main goal of the research was to explore the existence of phytochemicals, quantification of primary and secondary metabolites of leaves extract of Artabotrys hexapetalus (L.f.) Bhandari. The phytochemical activity of leaves of Artabotrys hexapetalus was assessed using different solvent extracts like water, ethanol, acetone, chloroform and petroleum ether. Among the different solvent extracts, aqueous leaves extract revealed the high content of phytochemicals. So the aqueous leaves extract was used for further investigations. Aqueous leaves extract of Artabotrys hexapetalus was subjected to quantitative analysis of primary metabolites like carbohydrates, proteins and amino acids. Quantitative analysis of secondary metabolites like flavonoids, tannins and phenols were performed using aqueous leaves extract of Artabotrys hexapetalus. Qualitative screening of phytochemicals reported the existence of carbohydrates, amino acids, proteins, flavonoids, alkaloids, saponins, phenols, glycosides, tannins and diterpenes. Quantitative analysis showed the presence of carbohydrates (43.16 ± 1.0 mg/g extract), proteins (60.4 ± 0.88 mg/g extract), amino acids (19.33 ± 1.30  mg/g extract), flavonoids (28.3 ± 0.91 mg/g extract), tannins (24.53 ± 1.02 mg/g extract) and phenols (7.63 ± 0.85 mg/g extract). The present study concluded that aqueous leaves extract of Artabotrys hexapetalus as a potential source of phytochemicals, primary and secondary metabolites.