The medicinal plant Calpurnia aurea leaves act as an anti-inflammatory source of 5-Lipoxygenase / Cyclooxygenase-2 dual inhibitors

Tesfay Weletnsae; Tekleweyni Tadege; Kidu Hintsa; Gopalakrishnan V K; Zenebe Hagos; Berihu Tekluu; Kamalakararao K; Muthulingam M; Krishna Chaithanya K

Tesfay Weletnsae ⁽¹⁾ , Tekleweyni Tadege ⁽²⁾ , Kidu Hintsa ⁽³⁾ , Gopalakrishnan V K ⁽⁴⁾ , Zenebe Hagos ⁽⁵⁾ , Berihu Tekluu ⁽⁶⁾ , Kamalakararao K ⁽⁷⁾ , Muthulingam M ⁽⁸⁾ , Krishna Chaithanya K ⁽⁹⁾

(1) Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia, Ethiopia ,

(2) Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia, Ethiopia ,

(3) Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia, Ethiopia ,

(4) Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia, Ethiopia ,

(5) Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia, Ethiopia ,

(6) Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia, Ethiopia ,

(7) Department of Biochemistry, Adikavi Nannaya University, Rajahmundry, Andhra Pradesh, India, India ,

(8) Department of Zoology, Faculty of Science, Annamalai University, Chidambaram, Tamil Nadu, India, India ,

(9) Department of Chemistry, College of Natural and Computational Sciences, Aksum University, Axum, Ethiopia, Ethiopia

Issue
Vol. 11 No. (SPL 4) (2020): Volume 11 Issue (SPL 4)

Keywords:

Calpurnia aurea, Cyclooxygenase, 5-Lipoxygenase, Anti-inflammatory activity

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Abstract

Calpurnia aurea is a medicinal plant belonging to the family of Fabaceae. Traditionally in Ethiopia the leaves have been used for the treatment of diarrhea, leishmaniasis, swelling, cough and snake bite. Based on the traditional use the leaves of C. aurea might be a promising source for development of anti-inflammatory agents. The objective of the current research was to explore the 5- Lipooxygenase (5-LOX), Cyclooxygenase-1 & Cyclooxygenase- 2 (COX-1 & COX-2) inhibitory activity of inflammatory enzymes of organic leaves crude extract of C. aurea and its phytochemical analysis. The dried leaves powder of the C. aurea (100 g) was extracted with successive soxhlet extraction by solvents of low polarity (Petroleum ether) to a high polar solvent (Ethanol). These organic leaf extracts C. aurea were assessed for the in vitro anti-inflammatory activity by 5- Lipooxygenase, Cyclooxygenase-1 & Cyclooxygenase-2 inhibitory activities by ELISA method and further subjected to phytochemical screening. The ethanolic leaves extract of C.aurea showed significant 5-LOX inhibitory activity of 51.07% at 100 µg/ml with IC₅₀ of 95.30µg/ml and the C.aurea leaves acetone extract showed the selective COX- 2 index (2.35) inhibitory activity with an IC₅₀ of 200.75µg/ml. The current work revealed that the C. aurea leaf acetone extract (CALAC) is shows potential source for the isolation and development of 5-LOX and COX-2 dual inhibitory compounds for management of inflammatory related diseases and inflammatory cancers.