Effects of silver nanoparticles on the anti-oxidant status and histological alterations in different organs in Indian mud crab Scylla Serrata

Abstract

In medicine and many other fields, the use of silver nanoparticles is inevitable. The negative impacts of silver accumulated silver nanoparticles on the environment and organisms need to be taken into consideration. The studies on the effect of silver nanoparticles on bio-indicators such as marine invertebrates pave the way to regulate the usage of silver nanoparticles. In the present study, the effect of silver nanoparticles on the hepatopancreas of Indian mud crab, Scylla serrata was studied. A 20 ppm of silver nanoparticles was exposed to S. serrata for ten days period. The morphological changes, the biochemical markers from hepatopancreas, histological analysis for hepatopancreas, gills and reproductive organs were examined. On the 2nd day exposure of silver nanoparticles showed the increased value of anti-oxidants and no morphological changes. But the 10th-day exposure shows the significantly reduced level of biochemical markers in the hepatopancreas and morphological changes were observed. The apoptotic cells and necrotic cells were recorded in hepatopancreas. Moreover, the cell architecture of gill tissues and reproductive tissues were observed. The silver nanoparticles in the concentration of 20 ppm caused significant changes in the hepatopancreas of S. serrata for ten days of exposure. The mechanism of silver nanoparticles needs to be studied to understand and protect models from the nanoparticles.