Effect of musa paradisiaca l. extract against hyperammonemia in rats

Abstract

This investigation was designed to determine the possible protective effect of root stock of Musa paradisiaca l extract (MP) against ammonium chloride (NH4Cl) induced hyperammonemia. Experimental hyperammonemia was induced in adult male Wistar rats (180–200 g) by intraperitoneal injections of NH4Cl (100 mg/kg body weight) thrice a week. Rats were treated with MP (300 mg/kg body weight) via oral administration. At the end of experimental duration, blood ammonia, plasma urea, serum liver marker enzymes and lipid peroxidation in plasma and tissues (liver and brain) of normal and experimental animals were analysed. The results revealed that NH4Cl induced hyperammonemia, as evidenced by increases in the levels of blood ammonia, plasma urea, lipid peroxidations such as thiobarbituric acid reactive substances (TBARS), hydroperoxides (HP) and activities of liver markers (alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) and the activities of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase and reduced glutathione (GSH), were decreased in liver and brain tissues of NH4Cl group compared with the normal group. On the other hand, MP treatment reversed all these biochemical indices. The study shows wider evidence for the antihyperammonemic, hepatoprotective, neuroprotective and antioxidant activities of MP extract against NH4Cl induced hyperammonemia.