Protective Effect of Daidzein on TNBS Induced Acute Ulcerative Colitis on Rats

Abstract

The present study aimed at investigating the potential protective effect of Daidzein on 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced acute ulcerative colitis in rats. Animals were treatment with TNBS 20 mg, TNBS dissolved in 50% ethanol by single intra-colonic application into the descending colon, Daidzein 40 and 80 mg/kg per oral, and standard sulfasalazine (SSZ) 360 mg/kg. for Two weeks. Colon was removed, length (CL), weight (CW), microscopic index (MI) processed for histopathological evaluation and estimation of oxidative colon marker contents of active lipid peroxidation (MDA) myeloperoxidase (MPO), reduced glutathione (GSH). Enzymatic activity of superoxide dismutase (SOD) and serum nitrate levels were assessed. TNBS induced significant (p < 0.001), increase in CW, MI, oxidative marker MDA, MPO, and serum nitrate content, TNBS induced a significantly decrease in CL, SOD, and GSH content. Treatment of Daidzein 40 and 80mg with TNBS decreased preserved colon parameter and histology close to normal, increased (P<0.001) SOD, CAT, GSH and TNF- α IL-6 and IL-8 down-regulate the levels to compare SSZ and Daidzein. Daidzein 40and 80mg restored TNBS-induced colon injury via inhibition of oxidative stress. Daidzein found to protect the TNBS Induced Acute Ulcerative Colitis in rats.