Evaluation of in vitro antioxidant and anti-atherogenic properties of selected Siddha polyherbal decoctions

Abstract

This research work explains the antioxidant and anti-atherogenic effects of selected Siddha polyherbal decoctions such as Nilavembu kudineer, Kabasura kudineer, Notchi kudineer  and Adathodai kudineer.  Even though all the above decoctions have been used for fever, cold, cough, bronchitis, dysphonea and body pain in Siddha system of medicine, their antioxidant and anti-atherogenic properties were not investigated scientifically. We have analyzed the polyphenolic content, antioxidant and anti-atherogenic properties of decoction of the above-mentioned herbal formulations. The toxicity of the decoctions was also performed in PMBC. In the investigated formulations, Notchi kudineer showed higher level of total phenolic content (560 mg GAE/100 g), followed by Adathodai kudineer (260 mg GAE/100 g), Nilavembu kudineer (150 mg GAE/100 g) and Kabasura kudineer (90 mg GAE/100 g). Similarly, Notchi kudineer exhibited strong antioxidant activity in terms of radical scavenging potential against DPPH (IC-50: 2.12 mg/L), followed the Adathodai kudineer (IC-50: 2.27 mg/L), Nilavembu kudineer (IC-50: 4.48 mg/L) and Kabasura kudineer (IC-50: 9.29 mg/L).  In toxicological study all the decoctions of selected formulations didn’t show any toxicity. Similarly, Nilavembu kudineer showed maximum inhibition of lipid peroxidation when compared to other herbal decoctions. Among the investigated Siddha formulations, Nilavembu kudineer was found to possess high antioxidant and anti-atherogenic potentials and hence it could be further investigated as anti-atherogenic drug using in vivo model.