Antimicrobial and hepatoprotective effect of ISM Drug in alcohol induced liver damage on Zebrafish larvae

Abstract

Liver cirrhosis occurs due to the extracellular matrix proteins accumulation which distorts the hepatic structure. The aim of our study is to investigate the antimicrobial and hepatoprotective effect of India Siddha Medicine(ISM) drug in alcohol-induced liver damage on zebrafish larvae. ISM drug, a hepato protective agent comprising of 15 herbs offers numerous formulations with advantages of hepatocellular rejuvenation has proven to be the most effective treatment to suppress liver damage. A series of analyses were carried out using alcohol-induced liver damage through herbal-based therapeutic drug and agar well diffusion method. For the hepatoprotective study, the zebrafish larvae were exposed to different concentrations of ethanol % (0.01, 0.05, 0.1, 0.5) and the treatment with 217.2 µg/ml of ISM drug has been evaluated. The outcome revealed the effect of ISM on alcohol-induced zebrafish larvae with a substantial increase in the percentage of viability. ISM drug showed antibacterial and antifungal activity in the diffusion method with a maximum zone of inhibition observed as 12 mm and 13.5 mm, respectively. Taken together, these results showed that the drug has hepatoprotective and antimicrobial activity which can be used for the treatment of liver cirrhosis.