Solvent displacement as emerging technique for norfloxacin loaded nanoparticulate suspension for ocular drug delivery

Abstract

The purpose of the present investigation was to design and evaluate norfloxacin (NRFN) loaded nanoparticulate suspension by solvent displacement technique. Prepared nanoparticulate suspensions were characterized by melting point, FTIR, DSC, TEM, particle size analysis, surface charge measurement, In vitro drug release and stability studies. The optimized nanoparticulate suspension (D1) showed an average particle size (100.7 nm), zeta potential (21.3 mV), high entrapment efficiency (92.13±0.16 %) and drug content (94.46±0.12 %). The invitro drug release of the optimized nanoparticulate suspension showed in the range of 72.16%, after 12 hrs. Stability study revealed that nanoparticulate suspension was more stable at room and refrigerator condition with slight change in particle size distribution after 1month. The release of optimized formulation was found to fit with Higuchi, ze- ro order model and the formulation released by non fickian diffusion mechanism and produce controlled release. These preliminary results indicates that norfloxacin loaded eudragit-RLPO nanoparticulate suspension could be effective in the treatment of ocular disease.