Screening of oil polluted soil and detection of biologically active agents which are helpful in drug formation

Abstract

In this present study, an effort has been made to identify the Physical properties (porosity of soil, density of soil, color of soil, texture of soil etc.), Chemical properties (organic matter, soil pH, salinity etc.) and biological properties (presence of microbial community) of polluted soil. Also, presence of some biological active compounds has been obtained from the polluted soil. The separation and identification of organic compounds present in polluted soil was done by using Column chromatography technique and Gas Chromatography-Mass Spectroscopy (GC-MS). GC-MS results demonstrated the presence of Heptane, 3-methyl-, Cyclohexane, 1,1-dimethyl-, Octane, 2-methyl-, Nonane, 3-methyl-, Tetratriacontane, pentacosnae, Octane, 3,6-dimethyl-, Decane, 4-methyl-, Tetradecane, 5- methyl-, Pentadecane, 3-Methyl-, 6-Tridecene, 2,2,4,10,12,12-hexamethyl-7-(3,5,5-trimethylhex, Heptadecane, 2,6,10,15-tetramethyl-, Beta-Sesquiphellandrene. These compounds have been found with antibacterial, antimicrobial, antiviral, antifungal properties. The molecular identification of isolated microorganisms (Isolate 1 -5) by 16s RNA sequencing confirmed the presence of Brevibacillus bortelensis, Rhodococcus rhodhochorus and three microbes were identified as Stenotrophomonas acidaminiphilia. From the results it have been concluded that the researcher has tried to explore the properties of untouched habitat soil of Rajasthan to find out such compounds which have applications in drug formation. Moreover getting such compounds from polluted source makes it more interesting.