In-vivo Antioxidant Activity of Ethanol Extract of Oldenlandia corymbosa (Linn.) on Streptozotocin with Nicotinamide induced Oxidative Stress in Wistar Rats

Abstract

The study was done to investigate the in-vivo antioxidant activities of the ethanol extract of the aerial part plant Oldenlandia corymbosa in diabetes induced rats with streptozotocin-nicotinamide (STZ with NA). Acute toxic study carried out OECD guideline 420 under this guide line followed the LD50 value fixed 2000mg/kg. This study was conducted for 21 days by treating the animals with plant extract and then induced oxidative stress by administering a single dose of streptozotocin 45mg/kg along with nicotinamide 110mg/kg (p.o). After administration of 100mg/kg and 200 mg/kg (p.o) of ethanol extract of Oldenlandia corymbosa (ETOC) to animals, the levels of hepatic and renal GSH, GST, GPx, SOD, CAT and nitric oxide, Thiobarbituric acid reactive substance (TBARS), hydroperoxides (HP), enzymes levels were estimated. The study proves Oldenlandia corymbosa (Linn.)’s significant in-vivo antioxidant activity helps protect tissues from oxidative stress and decreases a significant amount of hepatic and renal hydroperoxides and thiobarbituric acid substances. A considerable enhance in kidney and liver GSH, SOD, GPx, GST, CAT and NO when compared with diabetic control group was observed in the results. By administering ethanol extract of Oldenlandia corymbosa (Linn.) the dose level fixed 100mg, 200 mg/kg, (p.o) and glibenclamide (0.5 mg/kg) the GSH, GST, SOD, GPx, CAT levels considerably enhanced in comparison with diabetic control group. Hence this study concludes that ethanol extract of Oldenlandia corymbosa (Linn.) have considerable in-vivo antioxidant activity of aids in protecting tissue starting oxidative stress.