Investigation of antioxidant and antidiabetic activities of Murraya koenigii leaves methanolic and aqueous extract by in-vitro methods

Abstract

Phytochemical investigation of aqueous (AEMK) and methanolic (MEMK) extracts of Murraya koenigii were screened for the presence of phytoconstituents like phenolics, flavonoids, saponins, alkaloids, and glycosides. The antioxidant and antidiabetic activity of methanolic and aqueous extracts of Murraya koenigii leaves was estimated by DPPH radical scavenging assay and yeast glucose uptake method. Total phenolic and total flavonoid contents were quantitatively estimated. Total phenolics content in AEMK and MEMK were 2.214 mg/g and 2.591 mg/g respectively. Total flavonoid contents in AEMK and MEMK were 2.737 mg/g and 5.248 mg/g correspondingly. AEMK and MEMK have shown the remarkable DPPH free radical scavenging activity. Glucose uptake rate was dependent on the concentration of extracts and decreased the extracellular glucose concentration. In 300 µL concentration, methanolic and aqueous extracts of Murraya koenigii exhibited the highest antidiabetic activity in the yeast glucose uptake method. Thus, curry leaves showed a good natural source of antioxidative and antidiabetic compounds to prevent oxidative damage and to reduce blood sugar level.