Evaluation of antiulcer activity of organic grapes produced by microbial fertigation

Abstract

The investigation is aimed to evaluate the antiulcer activity of organic grapes produced by microbial fertigation and foliar Panchagavya. The gastric lesion was induced by oral administration of HCl - ethanol, and by pyloric ligation. The methanolic extract of grapes was administered at a dose of 100, and 200 mg/kg orally for five days before ulcer induction. Ranitidine was used as a reference standard. The antiulcer activity was assessed by determining and comparing the ulcer index in the test group with that of the standard drug- treated group. Gastric volume, total acid and free acid were estimated in the pylorus-ligated rats. The methanolic fruit extract (200 mg/Kg) showed maximum inhibition of gastric acid, free acidity and total acidity. The ulcer index of grape juice treated animals was found to be significantly less in all the models compared to standard drug-treated cases. The methanolic extract of Vitis vinifera(EVV) recorded higher ulcer inhibition rate against HCl – Ethanol-induced gastric lesion when compared with control and reported effective anti-secretory and antiulcer activity against pyloric ligation induced gastric ulcer in rats. Ulcer induced by aspirin plus ligated rats, pretreated with EVV showed a reduction in gastric acid secretion, free acidity, total acidity, ulcer score and increase in pH and ulcer inhibition rate when compared to control. The results showed the significant efficacy of fertigated grapes in antiulcer activity. The results were statistically analyzed by one- way ANOVA test and were found to be significant at p<0.05.