Expression of cholera toxin B antigen (CT-B) epitope in transgenic edible banana plant (Robusta sp.)

Abstract

Vibrio cholerae is a key purpose behind Cholera illnesses, and how to annihilate Cholera has been examined for a very long while. Oral antibodies delivered by transgenic plants would change the customary methods for generation and immunization of Cholera antibodies and lessen the inoculation cost essentially. In this examination interestingly we utilized the transgenic Banana (Robusta sp) framework to express Vibrio cholerae epitope immunization CTB, a recombinant peptide which could secure Cholera contamination after oral inoculation by expanding the particular antibodies. CTB was changed into Banana plant interceded by  Agrobacterium  tumifacience LBA4401, and transgenic shoots have been confirmed by PCR investigation, Western blot and ELISA examination demonstrated that CTB quality could transcript and create the objective peptide in the Leaf and Fruits. Transgenic Banana plant and their parts like leaf and Fruit product inferred oral antibody procedure could be conceivably uti- lized as an option system to battle for the Cholera contamination, particularly for the general population in re- mote and non-developed country.