Identify the Interleukin-6 gene -597 A/G Polymorphism in Type 2 diabetic patients

Abstract

Tumor necrosis factor-alpha (TNFa), and interleukin-6 (IL-6) are a cytokine which has a role in a metabolic progression which might facilitate diabetes progression. The current study was aimed to investigate the presence of Interleukin-6 gene -597 A/G Polymorphism in blood samples of 64 diabetic and 30 blood samples healthy control in Al-Diwaniyah City, Iraq. Using PCR RFLP, the results have shown that IL-6 polymorphism was pronounced in the patients with type 2 diabetes, particularly the genotype AA, GG, and AG and it showed s significant association with sex and age parameter. Also, this study found that the prevalence (Carriage rates) of allele A was is much lower than that in (Carriage rates) allele G in T2DM population compared to healthy population, which is confirmed the previous results. The current data will provide a sight on the role of heterogeneity of cytokine gene in the development of T2DM. Furthermore, these cytokine genes in addition of its variants might be a potential indicator for diseases susceptibility in south Iraq.