Influence of Diallyl disulphide on Hepatic Gluconeogenesis suppression by CREB Binding protein phosphorylation

Abstract

Diabetes is an important human ailment affecting many lives in different countries. Diallyl disulfide (DADS), the antidiabetic compound found in garlic, acts as a therapeutic agent in diabetes mellitus condition. This research aimed to investigate the role of DADS on the gluconeogenic mechanism in the liver tissue and the potential involvement of CREB in glucose homeostasis in a Wistar rat model. The alteration in the body weight, liver weight and glycogen content in diabetic rats were prevented by this therapeutic compound: the cAMP-responsive element-binding protein (CREB), an important transcriptional regulator of the gluconeogenic mechanism. The glucose uptake potential was studied by the expression of CREB protein in DADS treated diabetic rats using the western blotting technique. A high level of hepatic CREB protein expression was noted in diabetic status in the chronic hyperglycemic model which was reversed by DADS. The antihyperglycemic effect of DADS was almost similar to that of known antidiabetic drug metformin. The therapeutic action of DADS on diabetic status is due to the control of the glycemic imbalance in liver tissue.