High Fat Diet and Low Dose Streptozotocin Induced Diabetic Dyslipidaemia and Hepatic Damage in Rats

Abstract

The objective of the present study was to validate a rat model of diabetic dyslipidaemia along with hepatic damage by High fat diet and low dose streptozotocin combination that resembles the natural history of metabolic syndrome in humans. Male and Female Sprague Dawley rats were divided into 2 groups. One group of rats fed with high fat diet and other group was fed with normal pellet diet for a period of 2 weeks followed by streptozotocin administration on 15^th day. Body weight of the animals was measured on day 0, 14 and 21, also post 1 week of the streptozotocin administration the blood glucose, serum lipid, liver, kidney markers and insulin levels were measured. The high fat diet fed animals had exhibited a significant increase in body weight post two weeks of HFD feeding, but the body weight was reduced post 1 week of streptozotocin intervention due to metabolic disruption caused by STZ. The HFD group animals exhibited significant increase in blood glucose, serum total cholesterol, triglycerides, LDL, VLDL. Similarly, a significant decrease in HDL and serum insulin levels were observed as compared to the NPD fed animals. Further the HFD group animals had shown significant elevation of the liver function enzymes such as ALT and ALP levels as compared to the NPD fed animals. The present study confirms the experimental induction of diabetic dyslipidaemia along with hepatic damage which serves as an ideal model for metabolic syndrome along with hepatic cirrhosis which would useful for evaluating the therapeutic agents against type 2 diabetes, hyperlipidaemia and hepatic cirrhosis.