Abstract
Isolation of bioactive compounds on pekoe fanning black tea was done. The isolation was guided by xanthine oxidase (XO) inhibitory activity test. On the fractionation and separation steps, the ethyl acetate fraction and subfraction (SB)-3 had greater on XO activity with percentage of inhibition 71.38% and 74.49% respectively. The purification steps obtained four compounds and they were identified by spectral data of UV-Vis, 1H-NMR, MS, and 13C- NMR. From spectral data the compounds were known as kaempferol, quercetin, luteolin and myricetin. All isolates were assayed by XO inhibitory and their activity on interferon-γ (IFN-γ) production in vivo. Luteolin demonstrated the greatest of XO inhibitory activity with IC50 5.06 µg/ml. On IFN-γ production indicated that quercetin demonstrated the greatest ability with IFN- γ production 14.45 pg/ml.
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