Purification and characterization of Riboflavin carrier protein from egg yolk of South Indian spotted owlet (Athene brama)

Karunakar Rao Kudle (1) , M.P.Pratap Rudra (2)
(1) Department of Biochemistry, Osmania University, Hyderabad-500 007, Andhra Pradesh, India, India ,
(2) Department of Biochemistry, Osmania University, Hyderabad-500 007, Andhra Pradesh, India, India

Article Sidebar

Issue
Vol. 4 No. 4 (2013): Volume 4 Issue 4
Keywords:
    Spotted Owlet Eggs Yolk, DEAE-Sepharose, Sephdex G-100, fluorescence spectra, Circular dichroism (CD) spectroscopy
PDF

Abstract

Riboflavin carrier protein has been isolated and purified from the Indian spotted owlet (Athene brama). Purification could be achieved by DEAE-Sepharose column chromatography and gel filtration chromatography on Sephadex G-100. Further the RCP was immunologically characterized and compared with the hen (Gallus gallus domesticus) egg yolk RCP (RfBP). The protein was characterized using absorption, fluorescence and CD spectral analysis. Comparison of the mobility of the purified proteins with the standard molecular weight marker proteins revealed that the spotted Owlet egg RCP had a molecular weight close to 29.2kDa and it was approximately 3 kDa less than the hen egg Yolk RCP.

Full text article

Generated from XML file

Authors

Karunakar Rao Kudle
Department of Biochemistry, Osmania University, Hyderabad-500 007, Andhra Pradesh, India
M.P.Pratap Rudra
Department of Biochemistry, Osmania University, Hyderabad-500 007, Andhra Pradesh, India
mpprataprudra@gmail.com (Primary Contact)
Karunakar Rao Kudle, & M.P.Pratap Rudra. (2013). Purification and characterization of Riboflavin carrier protein from egg yolk of South Indian spotted owlet (Athene brama). International Journal of Research in Pharmaceutical Sciences, 4(4), 580–585. Retrieved from https://ijrps.com/home/article/view/3748
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX

Article Details

No Related Submission Found