Attenuation of whole cell of Salmonella sp., isolated from a rural community pond water and its appliances in rat model: A preliminary study

Abstract

There is need for acknowledgment of specific Ag (s), so that a vaccine candidate may be recognized against the dreaded strains like Salmonella sp. the causal organism for Typhoid. We have taken an effort to screen an isolated bacterial strain isolated from village pond water for its immune modulating activity. Four groups of mice were taken as a test animal model. Group I was taken as control, Group II was symptomatic; Group III was symptomatic, induced with supernatant of Salmonella culture and attenuated with neutral phosphate buffer formalin, while Group IV was symptomatic but induced with crude attenuated whole cells. The rat groups were kept for 28 days with periodical observation and were subjected to Total Leucocyte Count/ mm³. The group I, which were found to be healthy with an initial TLC count 7269/mm³ at the day 1 which could be maintained till the end of the day 28 (7648 mm³). Group II and Group III could not maintain the TLC count after day 14. There was a drastic reduction in TLC counts, 5917 /mm³ for Group II and 5194 /mm³ for Group III. Group IV could maintain the TLC count from day 1 to day 28. Group II and Group III were unable to survive after day 21. The pond water isolated Salmonella sp. attenuated with neutral phosphate buffer formalin had an immune modulatory activity in symptomatic rat models, which could be evidenced from TLC/mm³ count.