Antioxidant and cytotoxic effects of methanolic extract of Salicornia brachiata L. in HepG2 cells

Abstract

The aim of this study was to determine the antioxidant and anticancer activities of methanolic extract of S.brachiata. In this study; total phenolic content was estimated, and free radical scavenging activity was measured by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) method, 2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS), Ferric Thiocyanate (FTC), and Thiobarbituric acid (TBA). The methanolic extract of S.brachiata was tested against HepG2 cells for its inhibitory effect of cell viability study was carried out by MTT assay. The extract showed highest DPPH inhibition percentage of 85% and ABTS inhibitory activity of 75% at 1000 μg/mL. The inhibition percentage of lipid peroxidation was evaluated by FTC and TBA Method. The results showed decreased cell viability and increased growth inhibition at a concentration dependent manner and altered the cell morphology. The inhibition percentage with regard to cytotoxicity was found to be 80% at 1000 μg/mL. The methanolic extract of S.brachiata showed better antioxidant and anticancer activity.