Study of the effect of exposure to Phytoestrogens (Genistein) and estradiol (17β- estradiol) in the level of gene expression (CYP19A1) and quantification of aromatase in female rats

Abstract

The current study was carried out to investigate the role of Phytoestrogens (genistein) in different concentrations and estrogens manufactured in tablets coated with or without progesterone at the level of gene expression of CYP19A1 and quantitative estimation of aromatase compared to control group. The results of the present study showed a significant increase in (P<0.05). In the level of quantitative estimation of serum aromatase enzyme and for all treatments compared to the control group (124.12). The highest level of the enzyme (322.00) in the group treated with genistein in combination with estradiol, while, the lowest rise in the level of aromatase enzyme (164.37) in the group treated with genistein combined with progesterone. There was also a significant decrease in the concentration of ribosomal RNA and all the treatments compared to the control group (1068.95), the Lower of RNA concentration was (373.70) in the group treated with genistein at a concentration of 50 mg | kg, While Less low concentration of RNA (1068.95) was observed in the groups treated with concurrent genistein with estradiol. The results of the present study indicate that there was a significant increase (P <0.05) in the level of gene expression of the aromatase enzyme in ovarian tissue and for all the treatments compared to control group (305.51). The highest level of gene expression of the enzyme (7.189) was observed in the groups treated with concurrent genistein with estradiol, While the lowest level of gene expression of aromatase (2.204) was observed in the groups treated with genistein combined with progesterone.