Chrysin protects mice liver and kidney from methandienone-induced oxidative stress, inflammation a multi-biomarker approach

Abstract

Methandienone is a medication that has wide uses by competitors and young people in gyms for fitness. Chrysin (CR) is a characteristic natural flavonoid that has to incorporate being a cancer prevention agent, anti-inflammatory and anti-oxidant agent. Mus Musculus mice were therefore used in the current work to test the effects of methandienone on vital physiological functions as expressed by the liver, renal function tests and oxidation activity. The reverse effects of chrysin were also tested for the treatment of methandienone in order to determine the possibility of lowering levels of side effects. The results showed clear changes in biochemical effects on liver and kidney functions as a result of treatment with methandienone were also evident; The treated mice showed a significant elevation in the concentrations of serum levels of the hepatic function enzymes (ALP, AST, ALT) and kidney functions parameters (urea and creatinine). Combined treatment of mice with (methandienone + 25 mg/kg chrysin) caused a significant reduction in urea level and activities of ALP, ALT and AST (p<0.05) as compared to treatment with methandienone alone. Treatment with (methandienone + 50 mg/kg chrysin) also resulted in a significant decrease in the renal level and ALP and AST activities as compared to methandienone-treated mice. The present study concludes that methandienone causes damages to the liver and the kidney, as indicated by elevated levels of their functional enzymes. This effect indicates it increases oxidative stress in these organs, possibly due to elevated production of free radicals.