Spectrophotometric determination of Chloroquine in bulk and pharmaceutical formulations

Abstract

Two simple, sensitive, precise, accurate and cost-effective spectrophotometric methods have been developed for the determination of Chloroquine in pure and dosage forms. Both Method-A and B are based on the formation of ion association complex with acid dyes Wool fast blue (WFB) and Bromo cresol green (BCG).which are extractable into chloroform phase. The colored chromogen for Method-A shows absorption maximum at 590 nm and linear within the limits50-250μg/mL.Method-B produced colored chromogen which is measured at 420nm. Beer’s law is obeyed in the concentration range of 50-250 μg/mL for the developed method. Different experimental parameters affecting the color development and stability of colored product are carefully studied and optimized. The developed methods could be successfully applied to pharmaceutical formulations. The results obtained are in good agreement with those obtained using official methods.