Molecular study of bacterial infection from hemodialysis patients in Wasit centre, Iraq

Abstract

This study was conducted at the College of Medicine – Wasit University in Cooperation with the Wasit centre for dialysis from the period of October - 2017 to February -2018. It has been carried out on 100 urine samples and 100 blood samples from 100 hemodialysis patients referred to the Wasit centre of dialysis from both sex [male and female]. This study showed that from both 100 urine samples, and 100 blood samples, 40% UTIs, and 36% bacteremia. The main bacterial isolates from urine samples represented Escherichia coli (40%), and Proteus merabilis (11.1), Staphylococcus aureus (12.5%), Entero cloacae (12.5%), Pantoea spp (2.5) Klebsiella pneumonia (11.1), while bacterial isolates from blood samples are Pseudomonas aeruginosa (33.33%), and Staphylococcus aureus (30%), Pasteurella mulocida (19.44%), Entero cloacae (11.11%), and Streptococcus spp (2.55%). Antimicrobial sensitivity for Pseudomonas aeruginosa appeared sensitivity for Amikacin, Ciprofloxacin, Ceftazidime, and imipenem while Pasteurella mulocida showed sensitivity with Ciprofloxacin, Imipenem, aztreonam, and nalidixic acid. Escherichia coli observed sensitivity with Imipenem, Amikacin, Cefepime, Ciprofloxacin, and Nitrofurantoin. Bacteremia can be seen in a significant number of hemodialysis patients, and Pseudomonas aeruginosa is the most universal organism isolated which is resistant to commonly used anti- biotics, a significant number of patients had asymptomatic urinary tract infection in which E.coli is the most frequent microorganism isolate by using culture and, bacterial isolates detected 100% by PCR technique.