A sort of validated Bioanalytical method developed for the estimation of Etoposide and Cisplatin in rat plasma by using two different advanced liquid chromatographic techniques like HPLC and UPLC and its application in bioequivalence studies
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Etoposide, Cisplatin, Bioanalytical, HPLC, UPLC
Abstract
An easy, quick, precise, active and reproducible reverse method high performance liquid chromatography (RP-HPLC), as well as Ultra pressure liquid chromatography (UPLC) unique technique, was developed for the bio-analytical method of Etoposide and Cisplatin with Oxaplatin as an internal standard. Chromatographic analysis was performed using HPLC was Waters Alliance e-2695, and UPLC was Waters Acquity UPLC by using x-bridge phenyl 150x4.6mm, 3.5µ column and therefore the mobile phase containing 0.1% triethylamine and acetonitrile at a ratio out of 60:40 v/v. The flow rate was 1 ml/min, and analytes were detected at 283 nm employing a photodiode array detector at ambient temperature in both liquid chromatographic systems. The proposed biological method was proved in both HPLC and UPLC with USFDA guidelines. USP tailing is 1.1, 1.02 for etoposide and 1.16, 1.05 for Cisplatin in HPLC and UPLC. USP plate count is 4794, 3884 for Etoposide and 9289, 14487 for Cisplatin. The calibration curve under accumulation set of 5-100 ng/ml of etoposide and 10-200 ng/ml of Cisplatin in both HPLC and UPLC. The accuracy of low, middle and high-level quality control samples are taken in 50%, 100% and 150% levels. Stability study was administered altogether conditions are bench top, wet extract and auto sampler, freeze-thaw, short term stability and in long term stability. This is the advanced technique established to the straightforward, economical, suitable, precise, accurate and stable method for the analysis of Etoposide and Cisplatin and study of its stability and phar- macokinetic studies using rat plasma.
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References
AD’yakonov, V., Dzhemileva, L. U., Dzhemilev, U. M. 2017. Advances in the Chemistry of Natural and Semisynthetic Topoisomerase I/II Inhibitors. Studies in Natural Products Chemistry, pages 21– 86.
Alfarouk, K. O., Stock, C.-M., Taylor, S., Walsh, M., et al. 2015. Resistance to cancer chemotherapy: failure in drug response from ADME to P-gp. Cancer Cell International, 15(1):71–71.
Beyzadeoglu, M., Ozyigit, G., Selek, U. 2014. Radiation therapy for head and neck cancers: A case-based review. Springer.
Bosch, F. X., de Sanjosé, S. 2007. The Epidemiology of Human Papillomavirus Infection and Cervical Cancer. Disease Markers, 23(4):213–227.
Carell, T., Kurz, M. Q., Müller, M., Rossa, M., Spada, F. 2018. Non-canonical Bases in the Genome: The Regulatory Information Layer in DNA. Angewandte Chemie International Edition, 57(16):4296–4312.
DeVita, V. T., Rosenberg, S. A., Lawrence, T. S. 2015. DeVita, Hellman, and Rosenberg’s Cancer: Principles & Practice of Oncology. Wolters Kluwer Health.
Ebell, M. H., Culp, M. B. B., Radke, T. J. 2016. A Systematic Review of Symptoms for the Diagnosis of Ovarian Cancer. American Journal of Preventive Medicine, 50(3):384–394.
Ferri, F. 2017. Ferris clinical advisor 2018 E-book 5 books in 1. page 1253. Elsevier in health sciences.
Garcia, R. S., Brage, M. I. Y., Moolhuyzen, E. G., et al. 2016. Functional and postoperative outcomes after preoperative exercise training in patients with lung cancer: a systematic review and meta analysis. Interactive Cardio Vascular and Thoracic Surgery, 23(3):486–497.
Halford, S. 2005. O6-methylguanine methyltransferase in colorectal cancers: detection of mutations, loss of expression, and weak association with G:C>A:T transitions. Gut, 54(6):797–802.
Hande, K. R. 1998. Etoposide: four decades of development of a topoisomerase II inhibitor. European Journal of Cancer, 34(10):1514–1521.
Kondola, S., Manners, D., Nowak, A. K. 2016. Malignant pleural mesothelioma: an update on diagnosis and treatment options. Therapeutic Advances in Respiratory Disease, 10(3):275–288.
Kumar, S., Chinnusamy, V., Mohapatra, T. 2018. Epigenetics of Modified DNA Bases: 5-Methylcytosine and Beyond. Frontiers in Genetics, 9:640.
Langhans, W. 2000. Anorexia of infection: current prospects. Nutrition, 16(10):996–1005.
Machowicz, R., Janka, G., Jedrzejczak, W. W. 2016. Your critical care patient may have HLH (hemophagocytic lymphohistiocytosis). Critical Care, 20(1):215.
Maris, J. M., Hogarty, M. D., Bagatell, R., Cohn, S. L. 2007. Neuroblastoma. The Lancet, 369(9579):2106–2120.
Moon, R. E. 2014. Hyperbaric oxygen treatment for decompression sickness. Undersea and Hyperbaric Medical Society, 41(2):151–158.
Omata, T., Kodama, K., Watanabe, Y., Iida, Y., et al. 2017. Ovarian teratoma development after anti- NMDA receptor encephalitis treatment. Brain and Development, 39(5):448–451.
O’Reilly, K. M. A., Mclaughlin, A. M., Beckett, W. S., Sime, P. J. 2007. Asbestos-related lung disease. American Family Physician, 75(5):683–688.
Oun, R., Moussa, Y. E., Wheate, N. J. 2018. The side effects of platinum-based chemotherapy drugs: a review for chemists. Dalton Transactions, 47(19):6645–6653.
Pasmant, E., Ballerini, P., Lapillonne, H., Perot, C., et al. 2009. SPRED1 disorder and predisposition to leukemia in children. Blood, 114(5):1131–1131.
Schneider, J. W., Dittmer, D. P. 2017. Diagnosis and Treatment of Kaposi Sarcoma. American Journal of Clinical Dermatology, 18(4):529–539.
Smalley, W., Ytter, C. F., Labra, A. C., Wani, S., et al. 2019. AGA Clinical Practice Guidelines on the Laboratory Evaluation of Functional Diarrhea and Diarrhea-Predominant Irritable Bowel Syndrome in Adults (IBS-D). Gastroenterology, 157(3):851– 854.
Sultan, R., Haider, Z., Chawla, T. U. 2016. Diagnostic accuracy of CT scan in staging resectable oesophagal cancer. Journal of the Pakistan Medical Association, 66(1):90–92.
van Osch, F., Jochems, S. H., van Schooten, F., Bryan, R. T., Zeegers, M. P. 2016. Quantified relations between exposure to tobacco smoking and bladder cancer risk: a meta-analysis of 89 observational studies. International Journal of Epidemiology, 45(3):857–870.
Yang, L., Dong, J., Jiang, S., Shi, W., et al. 2015. Red and Processed Meat Consumption Increase Risk for Non-Hodgkin Lymphoma: A PRISMA-Compliant Meta-Analysis of Observational Studies. Medicine, 94(45).
Yildiz, H., Van Den Neste, E., Defour, J. P., Danse, E., Yombi, J. C. 2020. Adult haemophagocytic lympho histiocytosis: a review. An International Journal of Medicine.
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