Anti-carcinogenic and cytotoxic effect of Carthamus oxycantha Safflower seed oil extract on RD cell lines

Abstract

The search for the story of anticancer drugs continues. The Agent that can kill cancer cells should don't kill the normal cells. This study includes the effect of active substances in Carthamus oxyacantha on RD malignant cell lines. RD malignant cell lines are Rhabdomyosarcoma cancer cell line which taken from Caucasian girl infected by cancer in pelvic at seven-year-old. Many of solvents used to extraction active compounds from Carthamus oxycantha, where prepared oily, aqueous solutions for compartment between them. Cytotoxicity assay, for evaluating the activity of these extracts is done on human cancer cell lines RD, by using eight concentrations (7.81, 15.62, 31.25, 62.5, 125, 250, 500, 1000) µg /ml for each extract, and 24, 48, and 72 hours of exposure time. The Results show a gradually cellular toxic effect of extracts on cell lines growth, with dose and time-dependent manner for all types of these cell lines. There are some differences in the effect of these extracts based on the extracts and cancer cell. Through the estimation of cytotoxic concentration that kills 50% of cells (CC50) for each extract and cell type, we found that aqueous extract was the most potent cytotoxic extracts for these cancer cell lines, followed by crude, crude oily extract respectively. RD cells were the most sensitive type of cell lines to these. Active compound Triterpene concreted in the flower and oil extracted oleic acid and linoleic acid will role antitumor activity.