Attenuating Effect of Vitamin E and Tinospora cordifolia on Bisphenol-A Induced Apoptosis in Goat Testis
Article Sidebar
-
Capra hircus, Amelioration, BPA, Tinospora cordifolia, catalase, Vitamin E
Abstract
During the present study effect of Vitamin E and Tinospora cordifolia on BPA induced goat (Capra hircus) testicular tissue was evaluated. Testicular tissue was exposed to varied concentrations of BPA, Vitamin E and Tinospora cordifolia in three experimental groups along with their specific control for 4 and 8hrs. In group I, the cultured tissue was exposed to three doses of Bisphenol-A viz. 0.01, 1.0 and 100 nM/ml. In group II, Vitamin E (0.1µM/ml) was supplemented along with BPA (0.01, 1.0, 100 nM/ml) and in group III Tinospora cordifolia extract (250 µg/ml) was added with BPA (0.01, 1.0, 100 nM/ml) in the culture media. With the increase in dose level as well as the exposure duration, the frequency of apoptotic cells increased significantly (p< 0.05), as revealed by acridine orange and methylene blue staining. BPA exposure also induced a decline in the antioxidant enzyme activity such as catalase, glutathione peroxidase and reduced glutathione. Vitamin E and Tinospora cordifolia resulted in a decrease in the percentage of apoptotic cells and an increase in levels of catalase, glutathione peroxidase and reduced glutathione activity was observed. Hence, it is concluded that BPA induced more damage at its higher concentration and the antioxidant Vitamin E and Tinospora cordifolia ameliorate the oxidative stress-induced changes, thereby suggesting the potential of Vitamin E and Tinospora cordifolia in counteracting the cellular damage induced by agents like BPA.
Full text article
References
Aebi, H. 1984. Catalase in vitro. Methods Enzymol, 105:121–126.
Aitken, R.J., Roman, S.D. 2008. Antioxidant Systems and Oxidative Stress in the Testes. Oxidative Medicine and Cellular Longevity, 1(1):15–24.
Boyd, A.R., Gunasekera, T.S., Paul, V., Attfield, K., Simic, S.F., Vincent, D.A., Veal 2003. A flow-cytometric method for determination of yeast viability and cell number in a brewery. FEMS Yeast Research, 3(1):11–16.
Burton, G.W., Traber, M.G. 1990. Vitamin E: Antioxidant Activity, Biokinetics, and Bioavailability. Annual Review of Nutrition, 10(1):357–382.
El-Beshbishy, H.A., Aly, H.A.A., El-Shafey, M. 2013. Lipoic acid mitigates bisphenol A-induced testicular mitochondrial toxicity in rats. Toxicology and Industrial Health, 29(10):875–887.
Erler, C., Novak, J. 2010. Bisphenol an Exposure: Human Risk and Health Policy. Journal of Pediatric Nursing, 25(5):400–407.
Fang, Y., Zhou, Y., Zhong, Y., Gao, X., Tan, T. 2013. Effect of vitamin E on reproductive functions and antioxidant activity of adolescent male mice exposed to bisphenol A. Journal of Hygiene Research, 42(1):18–22.
Fryer, M.J. 1992. The antioxidant effects of thylakoid Vitamin E (alpha-tocopherol). Plant, Cell and Environment, 15(4):381–392.
Gandhi, A., Sharma, R.K. 2017. Bisphenol-A induced damage in testicular structure and its amelioration by Vitamin E and Tinospora cordifolia. Der Pharmacia Lettre, 9(8):57–63.
Gold, L.S., Manley, N.B., Slone, T.H., Garfinkel, G.B., Ames, B.N., Rohrbach, L., Stern, B.R., Chow, K. 1995. Sixth plot of the carcinogenic potency database: Results of animal bioassays published in the General Literature 1989 to 1990 and by the National Toxicology Program 1990 to 1993. Environmental Health Perspectives, 103(Supple 8):3– 122.
Hassan, Z.K., Elobeid, M.A., Virk, P., Omer, S.A., ElAmin, M., Daghestani, M.H., AlOlayan, E.M. 2012. Bisphenol A Induces Hepatotoxicity through Oxidative Stress in Rat Model. Oxidative Medicine and Cellular Longevity, 2012:1–6.
Jayaganthan, P., Perumal, P., Balamurugan, T.C., Verma, R.P., Singh, L.P., Pattanaik, A.K., Kataria, M. 2013. Effects of Tinospora cordifolia supplementation on semen quality and hormonal profile in rams. Animal Reproduction Science, 140(1-2):47– 53.
Kabuto, H., Hasuike, S., Minagawa, N., Shishibori, T. 2003. Effects of bisphenol A on the metabolisms of active oxygen species in mouse tissues. Environmental Research, 93(1):31–35.
Kalia, S., Bansal, M.P. 2008. Diethyl maleate-induced oxidative stress leads to testicular germ cell apoptosis involving Bax and Bcl-2. Journal of Biochemical and Molecular Toxicology, 22(6):371–381.
Kalia, S., Bansal, M.P. 2009. Regulation of apoptosis by Caspases under oxidative stress conditions in mice testicular cells: in vitro molecular mechanism. Molecular and Cellular Biochemistry, 322(1- 2):43–52.
Kwolek-Mirek, M., Zadrag-Tecza, R. 2014. Comparison of methods used for assessing the viability and vitality of yeast cells. FEMS Yeast Research, 14(7):1068–1079.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1):265–275.
Moron, M.S., Depierre, J.N., Mannervik, V.C. 1979. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA) - General Subjects, 582(1):67–78.
Padma, V.V., Baskaran, R., Divya, S., Priya, L.B., Saranya, S. 2016. Modulatory effect of Tinospora cordifolia extract on Cd-induced oxidative stress in Wistar rats. Integrative Medicine Research, 5(1):48–55.
Prince, P.S.M., Menon, V.P. 2001. Antioxidant action of Tinospora cordifolia root extract in alloxan diabetic rats. Phytotherapy Research, 15(3):213–218.
Rawal, A., Muddeshwar, M., Biswas, S. 2004. Effect of Rubia cordifolia, Fagonia cretica linn, and Tinospora cordifolia on free radical generation and lipid peroxidation during oxygen-glucose deprivation in rat hippocampal slices. Biochemical and Biophysical Research Communications, 324(2):588–596.
Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G. 1973. Selenium: Biochemical Role as a Component of Glutathione Peroxidase. Science, 179(4073):588–590.
Sangeetha, M.K., Mohanapriya, C.D., Vasanthi, H.R. 2013. The anti-diabetic property of Tinospora cordifolia and its active compound is mediated through the expression of Glut-4 in L6 myotubes. Phytomedicine, 20(3-4):246–248.
Sharma, R.K., Gulati, A. 2013. Malathion Induced Changes in Catalase and Superoxide dismutase in Testicular Tissues of Goat in-vitro. International Journal of Pharmacy and Biological Sciences, 3(4):193–197.
Takahashi, O., Oishi, S. 2001. Testicular toxicity of dietary 2,2-bis(4-hydroxyphenyl) propane (bisphenol A) in F344 rats. Archives of Toxicology, 75(1):42–51.
Takahashi, O., Oishi, S. 2003. Testicular toxicity of dietarily or parenterally administered bisphenol A in rats and mice. Food and Chemical Toxicology, 41(7):1035–1044.
Tohei, A., Suda, S., Taya, K., Hashimoto, T., Kogo, H. 2001. Bisphenol a Inhibits Testicular Functions and Increases Luteinizing Hormone Secretion in Adult Male Rats. Experimental Biology and Medicine, 226(3):216–221.
Wang, C., Fu, W., Quan, C., Yan, M., Liu, C., Qi, S., Yang, K. 2015. The role of Pten/Akt signaling pathway involved in BPA-induced apoptosis of rat sertoli cells. Environmental Toxicology, 30(7):793–802.
Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.